WOW! Proof Radiofrequency (RF) & Laser Used To Remotely Control Nanoparticles To Manipulate Genes & Cellular Activity

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StandUp4Liberty Sun, 05/23/2021 - 00:13

I have copied some of the page here. First red flag is this is in collaboration with Rockefeller University. Remember the Rockefeller "Lock-Step" Agenda?

How many times have we been called crazy conspiracy theorists for suggesting that RF/5G and nanoparticles could be used with a vaccine? The iron nanoparticles are the source for people reporting magnetics sticking to the injection site after they get the Jab.

This study was done on mice and human liver cells It will be promoted in a positive light for opportunities in treating human disease. However, if they can manipulate genes for good, it is just a easy to hack your cells and cause harm with this technology.

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Category: Cell and Biomolecular Engineering
Remote control of gene expression/magnetogenetics

Magnetogenetics

In collaboration with Jeffrey Friedman at the Rockefeller University and Sarah Stanley at the Icahn School of Medicine at Mount Sinai, we are combining nanotechnology and bioengineering to demonstrate that external and internally, genetically-encoded nanoparticles can be used in vivo to remotely regulate cellular activity. Calcium channels are of great therapeutic interest due to their numerous and varied functions throughout the body. One important channel, transient receptor potential vanilloid 1 (TRPV1), has gained great interest throughout the literature since its discovery. Understanding the channel’s role as a nociceptor has led to the development of treatments for a wide variety of diseases (e.g. pain caused by shingles). In particular, we have demonstrated that nanoparticles conjugated to TRPV1 can be used to remotely activate it and mediate cellular activity, such as neuron action potentials, gene transcription, and protein production. We have shown that the channel can be manipulated remotely to regulate gene expression in mice1. This was achieved by decorating His6-tag modified TRPV1 channels (TRPV1His) with anti-His6 antibody-coated iron oxide nanoparticles (αHis6-IONPs) and subjecting them to a radiofrequency (RF) field (Figure MG-1). Upon exposure to the RF field, the IONPs activate the decorated channels and cause them to open, allowing calcium ion flux into the cytoplasm, which subsequently activates a gene under the control of a calcium-sensitive promoter. We tested the system in vitro in human embryonic kidney cells (HEK-293T) expressing TRPV1His and a bioengineered proinsulin gene under the control of a calcium promoter. Proinsulin levels were found to increase significantly when incubated with αHis6-IONPs and treated with RF (Figure MG-2). We also showed that exposure to RF stimulates insulin release from xenograft tumors and this lowers blood glucose in diabetic mice. These studies established the efficacy of a novel platform for using nanotechnology to remotely control cellular response.

Figure MG-1. TRPV1-IONP system. The IONP is covalently coated in anti-His6 antibodies allowing it to bind to the His6-tag on the channel. When subjected to RF waves, the nanoparticle activates the channel and causes it to open. This results in calcium ion flux into the cell and the subsequent expression of a calcium-promoted gene. [We would like to acknowledge UltraFlex Power Technologies for the custom RF induction system used to generate the RF waves in the in vitro and in vivo studies described herein.]

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Figure MG-2. Efficacy of TRPV1-nanoparticle system in HEK-293T cells. Upon activation by RF waves, the particles cause the channel to open and the subsequent calcium flux promotes gene transcription. This effect is shut down by the channel antagonist Ruthenium red.

Temporally regulating gene expression and cellular activity are invaluable for elucidating underlying physiological processes and could have therapeutic implications. Building upon our TRPV1-IONP system, we developed a genetically encoded system for remote regulation of gene expression by either RF stimulation or exposure to a magnetic field2. Intracellularly, ferritin binds, converts, and stores excess iron ions as superparamagnetic iron oxide nanoparticles. We thus introduced and constitutively produced GFP-tagged ferritin light and heavy chain dimer fusion protein, which integrates with endogenous ferritin light and heavy chain monomers to form chimeric GFP-tagged ferritin 24-mers. The ferritin nanoparticles associate with a camelid anti-GFP nanobody TRPV1 fusion protein (αGFP-TRPV1), allowing for transduction of noninvasive RF or magnetic fields into channel activation. This, in turn, initiates calcium-dependent transgene expression (Figure MG- 3). In mice with viral expression of these genetically encoded components, remote stimulation of insulin transgene expression by RF or magnetic field exposure lowers blood glucose (Figure MG-4). This robust, repeatable method for remote regulation in vivo may ultimately have applications in basic science, technology, and therapeutics.

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Figure MG-3. TRPV1-ferritin system. An anti-GFP camelid nanobody is expressed on the N-terminus of the TRPV1 and binds to a GFP-tag chimerically integrated into ferritin nanoparticle. When subjected to RF waves, the nanoparticle activates the channel and causes it to open. This results in calcium ion flux into the cell and the subsequent expression of a calcium-promoted gene.

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